Evaluation of the Escherichia coli threonine deaminase gene as a selectable marker for plant transformation
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چکیده
منابع مشابه
Recombineering with tolC as a Selectable/Counter-selectable Marker: remodeling the rRNA Operons of Escherichia coli
This work describes the novel use of tolC as a selectable/counter-selectable marker for the facile modification of DNA in Escherichia coli. Expression of TolC (an outer membrane protein) confers relative resistance to toxic small molecules, while its absence renders the cell tolerant to colicin E1. These features, coupled with the lambdaredgam recombination system, allow for selection of tolC i...
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Two distinctly different L-threonine deaminases are known to be formed in Escherichia coli. One of them participates in the biosynthesis of L-isoleucine from L-threonine and is susceptible to an end product inhibition by the former compound (1). The other one seems to participate in the catabolism of L-threonine under anaerobic conditions and is activated by adenosine 5’monophosphate (1, 2). Li...
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Cysteine has been shown to inhibit growth in Escherichia coli strains C6 and HfrH 72, but not M108A. Growth inhibition was overcome by inclusion of isoleucine, leucine, and valine in the medium. Isoleucine biosynthesis was apparently affected, since addition of this amino acid alone could alter the inhibitory effects of cysteine. Homocysteine, mercaptoethylamine, and mercaptoethanol inhibited g...
متن کاملevaluation of spirulina platensis resistance to different antibiotics to find a selectable marker for genetic transformation
background spirulina is one of the most profitable known microalgae in the world, which is used as food and superfood. in the other hand, spirulina is a useful source of healthy components. it seems that the spirulina is transformable so that the introduction of a selectable marker is needed. objectives the purpose of this study was to determine a suitable selection marker for spirulina platens...
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ژورنال
عنوان ژورنال: Planta
سال: 2004
ISSN: 0032-0935,1432-2048
DOI: 10.1007/s00425-003-1129-x